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- Title
Methods for the detection of beef and pork in foods using real-time polymerase chain reaction.
- Authors
Laube, I.; Spiegelberg, A.; Butschke, A.; Zagon, J.; Schauzu, M.; Kroh, L.; Broll, H.
- Abstract
Summary Two TaqMan™-polymerase chain reaction (PCR) systems have been developed which permit the detection of even minute amounts of beef and pork in processed foods. In these methods cattle-specific primers amplified a fragment from the phosphodiesterase gene having a length of 104 base pairs (bp), and the swine-specific primers amplified a fragment from the ryanodin gene having a length of 108 bp. Beyond this, a third TaqMan™-PCR system, developed on the basis of the detection of the myostatin gene, permits a reliable exclusion of false-negative results by detecting meat from a variety of mammals or poultry in the material to be tested.
- Subjects
POLYMERASE chain reaction; BEEF; PORK
- Publication
International Journal of Food Science & Technology, 2003, Vol 38, Issue 2, p111
- ISSN
0950-5423
- Publication type
Article
- DOI
10.1046/j.1365-2621.2003.00651.x