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- Title
Plasmodium berghei bio-burden correlates with parasite lactate dehydrogenase: application to murine Plasmodium diagnostics.
- Authors
Lata De, Sai; Stanisic, Danielle I.; Rivera, Fabian; Batzloff, Michael R.; Engwerda, Christian; Good, Michael F.
- Abstract
Background: The spectrum of techniques to detect malaria parasites in whole blood is limited to measuring parasites in circulation. One approach that is currently used to enumerate total parasite bio-burden involves the use of bio-luminescent parasites. As an alternative approach, this study describes the use of a commercial ELISA human parasite lactate dehydrogenase (pLDH) detection kit to estimate total parasite bio-burden in murine malaria models. Methods: The cross reactivity of pLDH in a commercial human malaria pLDH diagnostic kit was established in different components of blood for different murine malaria models. The use of pLDH as a measure of parasite bio-burden was evaluated by examining pLDH in relation to peripheral blood parasitaemia as determined by microscopy and calculating total parasite bio-burden using a bio-luminescent Plasmodium berghei ANKA luciferase parasite. Results: The pLDH antigen was detected in all four murine Plasmodium species and in all components of Plasmodium-infected blood. A significant correlation (r = 0.6922, P value <0.0001) was observed between total parasite bioburden, measured as log average radiance, and concentration of pLDH units. Conclusions: This high throughput assay is a suitable measure of total parasite bio-burden in murine malaria infections. Unlike existing methods, it permits the estimation of both circulating and sequestered parasites, allowing a more accurate assessment of parasite bio-burden.
- Subjects
PLASMODIUM berghei; MAMMAL parasites; PLASMODIUM genetics; LABORATORY mice; ENZYME-linked immunosorbent assay
- Publication
Malaria Journal, 2016, Vol 15, p1
- ISSN
1475-2875
- Publication type
Article
- DOI
10.1186/s12936-015-1027-2