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- Title
Genotyping of Candida albicans by Fragment Analysis of Microsatellites Combined with 25S rDNA and RPS-based Strategies.
- Authors
Adachi, Hidesada; Shimizu, Kazue; Hattori, Hisao; Tanaka, Reiko; Chibana, Hiroji; Takagi, Yuki; Tomita, Yasushi; Kanbe, Toshio
- Abstract
Because of its high discriminatory potential, fragment analysis of microsatellites has been frequently used for genotyping of Candida albicans at the strain level. In order to evaluate a genotyping system based on the fragment analysis of microsatellites combined with PCRs targeting 25S rDNA and RPS, 456 independent strains of C. albicans were subjected to genotype analysis using 4 microsatellite markers (CDC3, HIS3, CAI and CAIII), followed by 25S rDNA and RPS-based genotyping. The fragment analysis using CAI showed the highest discriminatory potential (DP=0.9782), followed by HIS3 (DP=0.8780). Using combined microsatellite markers, 456 C. albicans strains were divided into 384 genotypes (DP=0.9984). PCRs targeting 25S rDNA and RPS were performed to differentiate the strains that showed identical genotypes in the fragment analysis, resulting in 434 genotypes (DP=0.9996). The combined genotyping system showed high discriminatory power at the strain level, and therefore is useful for rapid genotyping in molecular epidemiological studies of candidiasis.
- Subjects
CANDIDA albicans; GENETIC research; GENOTYPE-environment interaction; MICROSATELLITE repeats; EPIDEMIOLOGY
- Publication
Japanese Journal of Medical Mycology, 2009, Vol 50, Issue 3, p167
- ISSN
0916-4804
- Publication type
Article
- DOI
10.3314/jjmm.50.167