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- Title
FREQUENCIES OF XENOBIOTIC METABOLIZING ENZYME POLYMORPHISM IN MALAY SCHOOL CHILDREN.
- Authors
Alshagga, Mustafa Ahmed; Mohamed, Norazlina; Shuid, Ahmad Nazrun; Syed Zakaria, Syed Zulkifli
- Abstract
Introduction: Recent knowledge on the genetic basis of individual metabolic variation has opened new possibilities for studies focusing on increased susceptibility to environmental cancer. In this study we determined xenobiotic metabolizing enzymes polymorphism i.e phase I Cytochrome P450 1A1 (Msp1), 2E1 (Dra1) and phase II Glutathione-S-transferase (GSTM3) and (GSTT1) genes in a Malay school children. Method: Genetic polymorphism of 119 Malay school children from two schools in Wilayah Persekutuan Kuala Lumpur was determined by using Polymerase Chain Reaction/ Restriction Fragment Length Polymorphism (PCR/RFLP). Results: Frequencies of CYP1A1 (Msp1) were 0.32, 0.46 and 0.22 for homozygous wild type, heterozygous and homozygous variant, respectively. CYP2E1 (Dra1) frequencies were 0.52, 0.41 and 0.07 for homozygous wild type, heterozygous and homozygous variant, respectively. While Phase II GSTM3 frequencies were 0.90, 0.09 and 0.01 for GSTM3 wild type (GSTM3*A), heterozygous (GSTM3*AB) and mutant homozygous (GSTM3*B) respectively. GSTT1 positive subjects were 0.82 while deleted gene subjects frequency was 0.18. Conclusion: This study has provided a basis for further screening of people who are exposed to carcinogen and at a risk to develope environmental cancer.
- Subjects
MALAYSIA; GENETIC polymorphisms; XENOBIOTICS; CYTOCHROME P-450; GLUTATHIONE transferase; MALAYSIANS; SCHOOL children; CANCER risk factors
- Publication
Malaysian Journal of Medical Sciences, 2007, Vol 14, p205
- ISSN
1394-195X
- Publication type
Article