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- Title
Dual silencing of DmCPD and DmGA20ox genes generates a novel miniature and delayed-flowering Dendranthema morifolium variety.
- Authors
Xie, Qiaoli; Chen, Guoping; Liu, Qin; Zhu, Zhiguo; Hu, Zongli
- Abstract
Chrysanthemums (Dendranthema morifolium) are one of the most economically important perennial flowering plants, with floricultural (cut flowers), ornamental crop (pot and garden flowers) and, for some cultivars, medicinal uses. Plant architecture is an important agronomic trait for plants with a high ornamental and economic value. In this study, two miniature-related genes, DmCPD and DmGA20ox, were cloned and their tissue-specific expression patterns were analyzed. The results showed that the two genes were both highly expressed in stems, mature leaves, and flowers, and that DmCPD was also highly expressed in pedicels. To generate miniature plants, an RNAi expression vector targeting both DmCPD and DmGA20ox was constructed and transformed into chrysanthemum plants. Smaller plant size and slower growth and development of flowers were observed in dual-silenced chrysanthemums. Brassinosteroid and gibberellin contents in leaves and flower buds of transgenic plants were significantly decreased. Furthermore, the expressions of brassinolide-, gibberellin-, and flowering-related genes were down-regulated by varying degrees in dual-silenced plants. These results suggest that DmCPD and DmGA20ox play important roles in plant architecture, and brassinolide and gibberellin are important hormones in controlling plant architecture. This miniaturization strategy provides an efficient approach for generating new varieties of ornamental plants and crops.
- Publication
Molecular Breeding, 2015, Vol 35, Issue 2, p1
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-015-0239-z