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- Title
In vivo conversion of triacylglycerol to docosahexaenoic acid-containing phospholipids in a thraustochytrid-like microorganism, strain 12B.
- Authors
Hidetoshi Okuyama; Yoshitake Orikasa; Takanori Nishida
- Abstract
The thraustochytrid-like microorganism, strain 12B, cultivated in peptone, yeast extract, and 8% (w/v) glucose in 50% (v/v) seawater, accumulated docosahexaenoic acid (DHA)-rich triacylglycerol (TAG) at 67% of total lipid. When these TAG-accumulated cells were cultivated in glucose-deficient medium, dry cell weight (3 mg per ml culture) increased approximately 3-fold relative to baseline but the TAG/total lipid decreased to 5%. At the same time, the amount of phospholipid (5 mg) per whole culture also increased 3-fold. Hence, phospholipid/total lipid increased from 13% to 67%. High levels of DHA (more than 50% of total) were maintained in phosphatidylcholine.
- Subjects
THRAUSTOCHYTRIALES; GLYCERIN; YEAST; PHOSPHOLIPIDS; LIPIDS; MICROORGANISMS; DOCOSAHEXAENOIC acid; LECITHIN; GLUCOSE
- Publication
Biotechnology Letters, 2007, Vol 29, Issue 12, p1977
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-007-9492-5