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- Title
Block ionomer complexes containing cationic antibiotics to kill intracellular Brucella melitensis in vitro.
- Authors
Pothayee, N.; Jain, N.; Vadala, T. P.; Johnson, L. M.; Mejia‐Ariza, R.; Sriranganathan, N.; Davis, R. M.; Riffle, J. S.
- Abstract
Brucellosis, caused by Brucella spp., is the most common zoonotic disease worldwide. It is difficult to eradicate because the pathogen resides partially within phagocytic host cells and the polar antibiotics that are recommended for treatment do not enter cells efficiently. Core-shell block ionomer complexes (BICs) carrying antibiotics in their cores were designed to transport these drugs into cells. Polyether-polyacrylate copolymers were condensed with cationic aminoglycoside antibiotics to form BICs with diameters of 170-340 nm in water. An anionic poly(acrylate- b-ethylene oxide- b-propylene oxide- b-ethylene oxide- b-acrylate) copolymer blended with a poly(ethylene oxide- b-acrylate) diblock was condensed with gentamicin to afford complexes containing up to 42 wt% of the antibiotic. The poly(propylene oxide) contributed hydrophobic interactions that enhanced stability of the complexes in aqueous media, whereas the hydrophilic blocks provided a steric brush to keep the structures dispersed. In vitro efficacy of the BICs to reduce intracellular Brucella was studied in murine macrophage-like cells. Significant bacterial reductions of 2.78 and 2.85 logs were obtained relative to only 0.75 logs for the free drug. This suggests that the BICs are efficient transporters of polar antibiotics into phagocytic cells. Copyright © 2011 John Wiley & Sons, Ltd.
- Subjects
ZOONOSES; ANTIBIOTICS; IONOMERS; POLYETHERS; POLYACRYLATES; ETHYLENE oxide
- Publication
Polymers for Advanced Technologies, 2012, Vol 23, Issue 11, p1484
- ISSN
1042-7147
- Publication type
Article
- DOI
10.1002/pat.2070