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- Title
Andrology: Co-culture with Vero cell monolayer maintains the motility of asthenozoospermic semen samples.
- Authors
Chen, Hsin-Fu; Ho, Hong-Nerng; Chen, Shee-Uan; Lien, Yih-Ron; Chao, Kuang-Han; Lin, Heng-Ru; Huang, Su-Cheng; Lee, Tzu-Yao; Yang, Yu-Shih
- Abstract
The clinical effectiveness of co-culture with Vero (Green monkey kidney) cell monolayer in maintaining the motility and viability of fresh asthenozoospermic semen (18 samples) and frozen–thawed semen with poor motility (motility fraction <50%) (15 samples) in a 24-h period was evaluated. Co-culture with Vero cell monolayer in human tubal fluid (HTF) medium for 24 h resulted in a statistically better maintenance of motility percentage ( < 0.005), mean amplitude of lateral head displacement (ALH) ( < 0.005), and mean track speed (VCL) ( < 0.05) than culture in HTF medium alone. However, these motility parameters (motility percentage, ALH, VCL) declined soon after removal of spermatozoa from the monolayer. Co-culture with Vero cell monolayer also maintained the viability percentage of these sperm samples (52% of the original value) after the 24-h period compared with culture in HTF medium alone (22% of the original) (52% versus 22%, < 0.05).It is concluded that Vero cell monolayer is effective in the maintenance of motility and viability of asthenozoospermic semen or frozen–thawed semen with poor motility.This co-culture system may be beneficial in enhancing the in-vitro performance of asthenozoospermic semen samples in the practice of assisted reproductive technology.However, its safety needs further evaluation.
- Publication
Human Reproduction, 1994, Vol 9, Issue 7, p1276
- ISSN
0268-1161
- Publication type
Article