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- Title
Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by cholestane-3β, 5α, 6β-triol
- Authors
Liu, Hongmei; Yuan, Lan; Xu, Shanjin; Wang, Kui
- Abstract
Abstract: The cellular and molecular mechanisms that mediate vascular calcification remain poorly understood. In our previous study, oxysterol cholestane-3β, 5α, 6β-triol (Triol) was shown to promote vascular smooth muscle cells (VSMCs) calcification. In this study, by using direct coculture, non-contact transwell coculture, and culture with conditioned media, we investigated the roles of endothelial cells (ECs) and macrophages in the regulation of VSMCs calcification in the absence or presence of Triol. In vitro calcification was induced by incubation of VSMCs with β-glycerophosphate. The results showed that ECs inhibited VSMCs calcification, as manifested by the reduction of calcium deposition in extracellular matrix. This effect of ECs on calcification was via the secreted soluble factors. Furthermore, the stimulation of ECs by Triol had no influence on ECs inhibition of calcification. On the other hand, macrophages promoted VSMCs calcification via the secreted soluble factors such as reactive oxygen species, which was further enhanced by Triol. Our results supported the roles for ECs and macrophages in vascular calcification, modulated by oxysterols in atherosclerotic plaque.
- Subjects
BIOMINERALIZATION; CALCIUM in the body; SMOOTH muscle; MACROPHAGES
- Publication
Cell Biology International, 2007, Vol 31, Issue 9, p900
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1016/j.cellbi.2007.02.009