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- Title
Studies on Cloning Expression and Characterization of β-Porphyranase from Marine Bacterium Wenyingzhuangia aestuarii.
- Authors
Feng Ruifang; Zhang Yuying; Shen Jingjing; Chang Yaoguang
- Abstract
A novel GH16 β-porphyranase gene porl6Z was cloned from marine bacterium Wenyingzhuangia aestuarii 0F219 and expressed in E. coli, and the biochemical properties and hydrolytic patterns of the recombinant protein Porl6Z_Wa were characterized. Porl6Z_Wa showed the maximum activity at 45 °C and pH 6.5 and keep stable at a wide pH range from 4.0 to 9.0. Porl6Z_Wa exhibited a cold-adapted property. Porl6Z_Wa was an endo-acting enzyme. The final products of Porl6Z_Wa were majorly composed of porphyran disaccharides L6S-G, with a minor portion of tetrasac-charides and hexasaccharides which were substituted by agarose moieties and (or) modified by methyl groups. Porl6Z_Wa could be utilized as a promising tool for degradation of porphyran and directional preparation of porphyran disaccharides, which would facilitate the investigation of porphyran polysaccharides.
- Publication
Journal of Chinese Institute of Food Science & Technology, 2022, Vol 22, Issue 2, p98
- ISSN
1009-7848
- Publication type
Article
- DOI
10.16429/j.1009-7848.2022.02.011