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- Title
Salmonella Typhimurium with Eight Tandem Copies of bla NDM-1 on a HI2 Plasmid.
- Authors
Song, Huijuan; Zou, Siyu; Huang, Yi; Jian, Cui; Liu, Weiyong; Tian, Lei; Gong, Lu; Chen, Zhongju; Sun, Ziyong; Wang, Yue
- Abstract
Carbapenem-resistant Salmonella has recently aroused increasing attention. In this study, a total of four sequence type 36 Salmonella enterica subsp. enterica serovar Typhimurium (S. Typhimurium) isolates were consecutively isolated from an 11-month-old female patient with a gastrointestinal infection, of which one was sensitive to carbapenems and three were resistant to carbapenems. Via antibiotic susceptibility testing, a carbapenemases screening test, plasmid conjugation experiments, Illumina short-reads, and PacBio HiFi sequencing, we found that all four S. Typhimurium isolates contained a blaCTX-M-14-positive IncI1 plasmid. One carbapenem-sensitive S. Typhimurium isolate then obtained an IncHI2 plasmid carrying blaNDM-1 and an IncP plasmid without any resistance genes during the disease progression. The blaNDM-1 gene was located on a new 30 kb multiple drug resistance region, which is flanked by IS26 and TnAs2, respectively. In addition, the ST_F0903R isolate contained eight tandem copies of the ISCR1 unit (ISCR1-dsbD-trpF-ble-blaNDM-1-ISAba125Δ1), but an increase in MICs to carbapenems was not observed. Our work further provided evidence of the rapid spread and amplification of blaNDM-1 through plasmid. Prompting the recognition of carbapenem-resistant Enterobacterales and the initiation of appropriate infection control measures are essential to avoid the spread of these organisms.
- Subjects
SALMONELLA typhimurium; PLASMIDS; MULTIDRUG resistance; MICROBIAL sensitivity tests; SALMONELLA enterica; INFECTION control
- Publication
Microorganisms, 2024, Vol 12, Issue 1, p20
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms12010020