We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Targeted Mutations Produce Divergent Characteristics in Pedigreed Sake Yeast Strains.
- Authors
Klinkaewboonwong, Norapat; Ohnuki, Shinsuke; Chadani, Tomoya; Nishida, Ikuhisa; Ushiyama, Yuto; Tomiyama, Saki; Isogai, Atsuko; Goshima, Tetsuya; Ghanegolmohammadi, Farzan; Nishi, Tomoyuki; Kitamoto, Katsuhiko; Akao, Takeshi; Hirata, Dai; Ohya, Yoshikazu
- Abstract
Modification of the genetic background and, in some cases, the introduction of targeted mutations can play a critical role in producing trait characteristics during the breeding of crops, livestock, and microorganisms. However, the question of how similar trait characteristics emerge when the same target mutation is introduced into different genetic backgrounds is unclear. In a previous study, we performed genome editing of AWA1, CAR1, MDE1, and FAS2 on the standard sake yeast strain Kyokai No. 7 to breed a sake yeast with multiple excellent brewing characteristics. By introducing the same targeted mutations into other pedigreed sake yeast strains, such as Kyokai strains No. 6, No. 9, and No. 10, we were able to create sake yeasts with the same excellent brewing characteristics. However, we found that other components of sake made by the genome-edited yeast strains did not change in the exact same way. For example, amino acid and isobutanol contents differed among the strain backgrounds. We also showed that changes in yeast cell morphology induced by the targeted mutations also differed depending on the strain backgrounds. The number of commonly changed morphological parameters was limited. Thus, divergent characteristics were produced by the targeted mutations in pedigreed sake yeast strains, suggesting a breeding strategy to generate a variety of sake yeasts with excellent brewing characteristics.
- Subjects
RICE wines; ISOBUTANOL; PLANT breeding; GENOME editing; GENETIC mutation; CELL morphology
- Publication
Microorganisms, 2023, Vol 11, Issue 5, p1274
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms11051274