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- Title
Effects of induced precocious puberty on cranial growth in female Wistar rats.
- Authors
Izquierdo, Antonio de Moraes; Mishima, Fernanda Danielle; Carrard, Vinícius Coelho; Farina, Marcos; Gonçalves Nojima, Matilde da Cunha
- Abstract
This investigation examined the effects of pharmacologically induced precocious puberty on cranial growth in Wistar rats. Forty-eight female newborn Wistar rats were divided into two groups: a control group (C) and an experimental group (E), with four subgroups of six animals each. The time interval from birth until sacrifice differed between the subgroups, and was set at 30, 60, 90, and 120 days. An intramuscular single dose (300 μg) of steroid hormone danazol was administered on day 5 after birth, as a means of inducing precocious puberty. Alizarin (2 mg/100 g) was administered to three animals in each subgroup three days prior to sacrifice. Body mass and dates corresponding to the beginning of the oestrous cycle were recorded. Craniometric measurements were undertaken. Histological analysis using light and fluorescence microscopy was then carried out to qualitatively and quantitatively evaluate the spheno-occipital synchondrosis and to visualize bone deposition patterns. The results were analysed with a Student's t-test and analysis of variance.Precocious puberty was effectively induced and differences between groups denoted an earlier maturation in the experimental rats. In qualitative analysis, a significant increase of total synchondrosis width was noted only in group E60, in comparison with C60, and an increase in the E90 subgroup cortical bone width compared with the C90 subgroup. Histomorphometrically, a statistical difference between total width values of subgroups E60 (434.3 μm) and C60 (323.5 μm) was detected. However, body mass and macroscopic measurements did not show statistically significant differences. An appropriate model for studying bone growth associated with precocious puberty in Wistar female rats was not achieved using steroid hormone danazol, when evaluated at 30 day intervals.
- Subjects
PRECOCIOUS puberty; CRANIAL nerves; RATS; ANIMAL models in research; QUALITATIVE research
- Publication
European Journal of Orthodontics, 2012, Vol 34, Issue 2, p133
- ISSN
0141-5387
- Publication type
Article
- DOI
10.1093/ejo/cjq130