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- Title
Direct Observation of a Central Bare Zone in a Native Thick Filament Isolated from the Anterior Byssus Retractor Muscle of Mytilus edulis Using Fluorescent ATP Analogue.
- Authors
Oiwa, Kazuhiro; Yamaga, Takako; Yamada, Akira
- Abstract
To investigate the existence of a central bare zone in native thick filaments isolated from the anterior byssus retractor muscle (ABRM) of blue mussels (Mytilus edulis), the filaments were observed by fluorescence and dark-field microscopy after being incubated in the presence of Ca2+ with the fluorescent ATP analogue, Cy3-EDA-ATP. Filaments appeared under dark-field illumination as thin rods with tapered ends of length 5–30 um. Fluorescence microscopy revealed that Cy3-EDA-ATP was bound to these filaments, except near their center. Although the boundary between this central non-fluorescent zone and fluorescent regions was not clearly defined, there was a trend for the width of the central nonfluorescent zone to increase with thick filament length (correlation coefficient=0.45; n= 142). When Cy3-EDA-nucleotides bound to thick filaments were displaced by excess ATP, fluorescent images disappeared with a rate constant of 0.024 s−1, close to the turnover rate of Cy3-EDA-ATP by myosin on the native thick filaments. These results indicate that each native thick filament isolated from the ABRM has a central bare zone, but its boundary was not sharply resolved.
- Subjects
MYTILUS edulis; LUMINESCENCE; FLUORESCENCE microscopy; NUCLEIC acids; RADIOACTIVITY
- Publication
Journal of Biochemistry, 1998, Vol 123, Issue 4, p614
- ISSN
0021-924X
- Publication type
Article
- DOI
10.1093/oxfordjournals.jbchem.a021981