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- Title
Heterogeneity of surface immunoglogulin on murine B lymphocytes.
- Authors
Parkhouse, R. M. E.; Hunter, I. R.; Abney, Erika R.
- Abstract
Surface IgM on murine splenic lymphocytes was capped using a rhodamine-coupled anti-μ chain system. The cells were then reacted with a polyspecific, fluorescein-coupled anti-mouse immunoglobulin (Ig) under non-capping conditions. A large number (31–41 per cent of total Ig-bearing cells) which gave red caps with anti-μ subsequently stained green peripherally with anti-Fab under the non-capping conditions. The remaining stained cells were divided between those showing only red caps (17–29 per cent of Ig-bearing cells) and those showing only green rings (37–45 per cent of Ig-bearing cells). These data are interpreted by assuming that most of the staining with the second reagent (fluorescein- coupled anti-mouse Ig) is due to the presence of surface associated IgD.
- Subjects
IMMUNOGLOBULINS; HETEROGENEITY; IMMUNOGLOBULIN M; LYMPHOCYTES; HOMOGENEITY; IMMUNOLOGY
- Publication
Immunology, 1976, Vol 30, Issue 3, p409
- ISSN
0019-2805
- Publication type
Article