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- Title
The Allergens of <em>Schistosoma mansoni</em> II. FURTHER SEPARATION BY SEPHADEX G-200 AND ION-EXCHANGE CHROMATOGRAPHY.
- Authors
Harris, W. G.
- Abstract
A total of thirty-five antigen fractions were prepared from adult Schistosoma mansoni by extraction into borate-buffered saline, precipitation at pH 4·6, gel-filtration on Sephadex G-100 and G-200, and ion-exchange chromatography on CM- and DEAE-cellulose. The allergic activity of these antigens was assayed by a modified Prausnitz-Küstner (P-K) type reaction in rats. The results showed that the allergen-reagin axis in rat schistosomiasis is a multi-component system involving molecules of widely different chemical nature and molecular weight, rather than a simple, single antigen-antibody interaction. The significance of these findings to the use of purified antigens for the diagnosis of schistosomiasis in the field is discussed.
- Subjects
BACTERIAL antigens; SCHISTOSOMA mansoni; ION exchange chromatography; SCHISTOSOMIASIS diagnosis; ALLERGIES; ANIMAL models of immunology
- Publication
Immunology, 1975, Vol 29, Issue 5, p935
- ISSN
0019-2805
- Publication type
Article