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- Title
Adipogenic Impairment of Adipose Tissue-Derived Mesenchymal Stem Cells in Subjects With Metabolic Syndrome: Possible Protective Role of FGF2.
- Authors
Oliva-Olivera, Wilfredo; Coín-Aragüez, Leticia; Lhamyani, Said; Clemente-Postigo, Mercedes; Alcaide Torres, Juan; Bernal-López, Maria Rosa; El Bekay, Rajaa; Tinahones, Francisco José; Torres, Juan Alcaide
- Abstract
<bold>Context: </bold>The decreased expansion capacity of adipose tissue plays a crucial role in the onset of disorders associated with metabolic syndrome.<bold>Objective: </bold>The aim of this study was to examine the state of adipose tissue-derived mesenchymal stem cells (ASCs) from obese subjects with different metabolic profiles.<bold>Design: </bold>This was a 2-year study to enroll subjects who underwent bariatric surgery or cholecystectomy.<bold>Setting: </bold>University Hospital.<bold>Patients and Intervention: </bold>Patients who underwent either bariatric surgery (20 morbidly obese) or cholecystectomy (40 subjects) participated in the study.<bold>Main Outcome Measures: </bold>ASCs were obtained from both visceral and subcutaneous adipose tissue. Adipogenic, fibrotic gene expression was quantified by quantitative polymerase chain reaction; Smad7 and fibroblast growth factor 2 were quantified by western blotting and enzyme-linked immunosorbent assay, respectively. The susceptibility of ASCs to apoptosis, their population doubling time, and their clonogenic potential were evaluated.<bold>Results: </bold>The worsening metabolic profile of the patients was accompanied by a decrease in the intrinsic levels of adipogenic gene expression, reduced proliferation rate, clonogenic potential, and exportation of fibroblast growth factor 2 to the cell surface of the ASCs derived from both tissues. In addition, the ASCs from patients without metabolic syndrome showed differences in susceptibility to apoptosis and expression of TGFβ-signaling inhibitory protein Smad7 with respect to the ASCs from patients with metabolic syndrome.<bold>Conclusion: </bold>Our results suggest that the decrease in adipogenic-gene mRNA and clonogenic potential, as well as the accumulation of fibrotic proteins with metabolic alterations, could be a relevant mechanism controlling the number and size of neogenerated adipocytes and involved in alteration of adipose-tissue expansion.
- Publication
Journal of Clinical Endocrinology & Metabolism, 2016, pjc20162256
- ISSN
0021-972X
- Publication type
journal article
- DOI
10.1210/jc.2016-2256