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- Title
Citrate induces apoptosis of the acute monocytic leukemia U937 cell line through regulation of HIF-1α signaling.
- Authors
XIAOWEI XU; BAIWEN LI; PEIJIE HUANG; XINJIAN WAN; YOUWEN QIN; LILI ZHOU; HUIXIA LIU; HAITAO BAI; YANRONG GAO; CHUN WANG; XIANGJUN MENG
- Abstract
The present study aimed to investigate the anti-tumor effect of citrate on acute monocytic leukemia (AML) and its mechanisms. The apoptosis of the AML cell line, U937, was assessed by MTT and Hoechst staining, the expression of Bcl-2, caspases-3 and -9, hypoxia-inducible factor 1α (HIF-1α) and its target gene GLUT-1, were assayed by western blotting and the role of HIF-1α was evaluated through siRNA. The results showed that citrate inhibits the expression of Bcl-2, while it induces the activation of caspases-3 and -9. In addition, citrate induces U937 apoptosis in a dose-and time-dependent manner by regulating the expression of HIF-1α and its downstream target GLUT-1. The results suggest that citrate performs an anti-acute monocytic leukemia action by targeting HIF-1α signaling and may be a promising clinical approach.
- Subjects
ANTINEOPLASTIC agents; MONOCYTIC leukemia; APOPTOSIS; CELL lines; GENE expression; CELLULAR control mechanisms
- Publication
Molecular Medicine Reports, 2013, Vol 8, Issue 5, p1379
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2013.1702