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- Title
Metabolomic study of serum in patients with invasive ductal breast carcinoma with LC-MS/MS approach.
- Authors
Amiri-Dashatan, Nasrin; Yekta, Reyhaneh Farrokhi; Koushki, Mehdi; Arefi Oskouie, Afsaneh; Esfahani, Hadi; Taheri, Salman; Kazemian, Elham
- Abstract
Background: Invasive ductal carcinoma (IDC) is the most common type of breast cancer so its early detection can lead to a significant decrease in mortality rate. However, prognostic factors for IDC are not adequate and we need novel markers for the treatment of different individuals. Although positron emission tomography and magnetic resonance imaging techniques are available, they are based on morphological features that do not provide any clue for molecular events accompanying cancer progression. In recent years, "omics" approaches have been extensively developed to propose novel molecular signatures of cancers as putative biomarkers, especially in biofluids. Therefore, a mass spectrometry-based metabolomics investigation was performed to find some putative metabolite markers of IDC and potential metabolites with prognostic value related to the estrogen receptor, progesterone receptor, lymphovascular invasion, and human epidermal growth factor receptor 2. Methods: An untargeted metabolomics study of IDC patients was performed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). The multivariate principal component analysis by XCMS online built a model that could separate the study groups and define the significantly altered m/z parameters. The most important biological pathways were also identified by pathway enrichment analysis. Results: The results showed that the significantly altered metabolites in IDC serum samples mostly belonged to amino acids and lipids. The most important involved pathways included arginine and proline metabolism, glycerophospholipid metabolism, and phenylalanine, tyrosine, and tryptophan biosynthesis. Conclusions: Significantly altered metabolites in IDC serum samples compared to healthy controls could lead to the development of metabolite-based potential biomarkers after confirmation with other methods and in large cohorts.
- Subjects
BREAST; PROGESTERONE receptors; LIQUID chromatography-mass spectrometry; DUCTAL carcinoma; EPIDERMAL growth factor receptors; METABOLOMICS; POSITRON emission tomography
- Publication
International Journal of Biological Markers, 2022, Vol 37, Issue 4, p349
- ISSN
0393-6155
- Publication type
Article
- DOI
10.1177/03936155221123343