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- Title
Hu antigen R (HuR) heterogeneous expression quantification as a prognostic marker of melanoma.
- Authors
Liaudet, Nicolas; Fernandez, Marylise; Fontao, Lionel; Kaya, Gürkan; Merat, Rastine
- Abstract
Background: Prognostic markers for melanoma, particularly for stage II disease, are needed for the risk‐benefit evaluation of future adjuvant therapies. The mainly nuclear RNA‐binding protein human antigen R (HuR) regulates the protein expression of thousands of mRNAs, its own heterogeneous expression could therefore reflect tumor heterogeneity and plasticity. Here, we evaluate its quantification in primary melanoma as a marker of metastatic outcome. Methods: We conducted an immunohistochemistry‐based automated quantification of HuR nuclear expression heterogeneity in primary melanomas, most with Breslow thickness ≥ 1 mm and calculated the dimensionless fourth moment, that is, the kurtosis of HuR (HuR K) expression distribution. Twelve tumors from patients with no metastatic disease were compared to a similar number of tumors from patients who had metastatic disease at 2 years follow up. Results: HuR K value appeared significantly higher in the non‐metastatic group comparatively to the metastatic group (<italic>P</italic> = 2.84 × 10−3, 1‐tailed Wilcoxon rank‐sum test). Moreover, compared to the Breslow thickness, HuR K value appeared as a more robust marker of metastatic outcome (respective areas under receiver operating characteristic curves 0.84 and 0.87). Conclusion: Our data need confirmation on a large cohort, however strongly suggest that HuR expression heterogeneity quantification using kurtosis, could be used as a prognostic marker in melanoma.
- Subjects
MELANOMA; MELANOMA diagnosis; PROTEIN expression; ANTIGEN analysis; RNA-binding proteins; PATIENTS
- Publication
Journal of Cutaneous Pathology, 2018, Vol 45, Issue 5, p333
- ISSN
0303-6987
- Publication type
Article
- DOI
10.1111/cup.13119