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- Title
Development of an immunochromatographic strip for the detection of antibodies against Porcine circovirus-2.
- Authors
Jin, Qianyue; Yang, Jifei; Lu, Qingxia; Guo, Junqing; Deng, Ruiguang; Wang, Yinbiao; Wang, Shihong; Wang, Shufen; Chen, Wen; Zhi, Yubao; Wang, Li; Yang, Suzhen; Zhang, Gaiping
- Abstract
A rapid (<5 min) immunochromatographic strip using a colloidal gold–labeled antigen probe was successfully developed and applied for the detection of Porcine circovirus-2 (PCV-2) antibodies in swine. Recombinant Cap protein truncated nuclear localization signal of PCV-2, was expressed and labeled with colloidal gold. This conjugate was dispensed on a conjugate pad as the detector. Staphylococcal protein A and purified porcine anti–PCV-2 antibodies were blotted on a nitrocellulose membrane for the test and control lines, respectively. Sensitivity and specificity of this strip test was evaluated using PCV-2 antisera as well as other sera from pigs infected with a variety of swine viruses. For the validation of this strip test, 500 clinical swine serum samples were assessed both by the strip and a commercial enzyme-linked immunosorbent assay (ELISA) kit. The agreement between the immunochromatographic strip and ELISA kit was 94.00%. This strip possesses high sensitivity and specificity and may be useful as a candidate for rapid diagnosis of PCV-2 antibodies in the field.
- Subjects
CIRCOVIRUSES; CHROMATOGRAPHIC analysis; IMMUNOGLOBULINS; SWINE; NITROCELLULOSE; STAPHYLOCOCCAL protein A; ENZYME-linked immunosorbent assay
- Publication
Journal of Veterinary Diagnostic Investigation, 2012, Vol 24, Issue 6, p1151
- ISSN
1040-6387
- Publication type
Article
- DOI
10.1177/1040638712462374