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- Title
Proteolytic processing of human prorenin in renal and non-renal tissues.
- Authors
Reudelhuber, Timothy L.; Ramla, Djamel; Chiu, Linda; Mercure, Chantal; Seidah, Nabil G.
- Abstract
Previous studies have demonstrated that the motise proprotein convertase PC1 (mPC1) accurately cleaves human prorenin to generate active renin and that this processing event appears to require co-packaging in secretory granules. In the current study, we have tested human PC1 (hPC1; also called PC3) for its ability to activate human prorenin. Our results suggest that while hPCl is capable of carrying out the specific cleavage of human prorenin, it docs so at a reduced efficiency as compared to mPC1. This difference is due to sequences in the carboxy-terminus of PC1 as demonstrated by the activity of hybrid hPC1/mPC1 molecules. These studies demonstrate that PC1 cleavage of prorenin can occur in humans and identify a functionally important region in the hPC1 protein for this interaction. Moreover, the localization of PC1 in human tissues suggests that it may participate in the generation of active renin in the adrenal medulla and possibly in certain adrenal tumors.
- Subjects
RENIN; GENES; ADRENAL medulla; ADRENAL tumors; MOLECULES; PROTEIN-protein interactions
- Publication
Kidney International, 1994, Vol 46, Issue 6, p1522
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1994.435