We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Integrin β-4 Signaling Plays a Key Role in Mouse Embryogenesis.
- Authors
Roberts, Jeffrey E.; Nikolopoulos, Sotiris N.; Oktem, Ozgur; Giancotti, Filippo; Oktay, Kutluk
- Abstract
Integrins, by signaling between extracellular matrix and cell nucleus, serve critical roles in cell proliferation and survival. A knock-in mice was developed by a targeted deletion of the C-terminal segment of the cytoplasmic tail of β4-integrin (β4-1355T). The β4-1355T mice had a longer gestational length, smaller litter sizes, lower fecundity rate, and higher frequency of early pregnancy loss. β4-1355T embryos demonstrated a high degree of fragmentation and asymmetry, with fewer surviving to either a morula or blastocyst stage. In wild-type oocytes and embryos, β1, β4, and laminin-5 signals colocalized at the opposing surfaces of blastomeres and between the polar bodies and oocytes. Blastomeres within the β4-1355T embryos were less cohesive, with a more diffuse expression of β4 and laminin-5 compared with wild type. The α6β4_laminin-5 interaction appears to be vital for maintaining the cohesiveness between the cells of the embryo. Deciphering the role of integrins such as β4 in embryogenesis may help explain in vitro fertilization failures.
- Subjects
INTEGRINS; EMBRYOLOGY; LABORATORY mice; EXTRACELLULAR matrix; CELL nuclei; CYTOPLASM; EMBRYOS
- Publication
Reproductive Sciences, 2009, Vol 16, Issue 3, p286
- ISSN
1933-7191
- Publication type
Article
- DOI
10.1177/1933719108325506