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- Title
Two non-allelic nuclear genes restore fertility in a gametophytic pattern and enhance abiotic stress tolerance in the hybrid rice plant.
- Authors
Huang, Wenchao; Hu, Jun; Yu, Changchun; Huang, Qi; Wan, Lei; Wang, Lili; Qin, Xiaojian; Ji, Yanxiao; Zhu, Renshan; Li, Shaoqing; Zhu, Yingguo
- Abstract
In indica rice, the HongLian (HL)-type combination of cytoplasmic male sterility (CMS) and fertility restoration ( Rf) is widely used for the production of commercial hybrid seeds in China, Laos, Vietnam and other Southeast Asian countries. Generally, any member of the gametophytic fertility restoration system, 50% of the pollen in hybrid F plants displays recovered sterility. In this study, however, a HL-type hybrid variety named HongLian You6 had approximately 75% normal (viable) pollen rather than the expected 50%. To resolve this discrepancy, several fertility segregation populations, including F and BCF derived from the HL-CMS line Yuetai A crossed with the restorer line 9311, were constructed and subjected to genetic analysis. A gametophytic restoration model was discovered to involve two non-allelic nuclear restorer genes, Rf5 and Rf6. The Rf5 had been previously identified using a positional clone strategy. The Rf6 gene represents a new restorer gene locus, which was mapped to the short arm of chromosome 8. The hybrid F plants containing one restorer gene, either Rf5 or Rf6, displayed 50% normal pollen grains with I-KI solution; however, those with both Rf5 and Rf6 displayed 75% normal pollens. We also established that the hybrid F plants including both non-allelic restorer genes exhibited an increased stable seed setting when subjected to stress versus the F plants with only one restorer gene. Finally, we discuss the breeding scheme for the plant gametophytic CMS/ Rf system.
- Subjects
NUCLEAR proteins; GAMETOPHYTES; PLANT hybridization; EFFECT of stress on plants; CYTOPLASMIC male sterility; LOCUS (Genetics); PLANT breeding
- Publication
Theoretical & Applied Genetics, 2012, Vol 124, Issue 5, p799
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-011-1755-9