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- Title
Ghrelin Agonist JMV 1843 Increases Food Intake, Body Weight and Expression of Orexigenic Neuropeptides in Mice.
- Authors
HOLUBOVÁ, M.; ŠPOLCOVÁ, A.; DEMIANOVÁ, Z.; SÝKORA, D.; FEHRENTZ, J. A.; MARTINEZ, J.; ŠTOFKOVÁ, A.; JURČOVIČOVÁ, J.; DRÁPALOVÁ, J.; LACINOVÁ, Z.; HALUZÍK, M.; ŽELEZNÁ, B.; MALETÍNSKÁ, L.
- Abstract
Ghrelin and agonists of its receptor GHS-R1a are potential substances for the treatment of cachexia. In the present study, we investigated the acute and long-term effects of the GHS-R1a agonist JMV 1843 (H-Aib-DTrp-D-gTrp-CHO) on food intake, body weight and metabolic parameters in lean C57BL/6 male mice. Additionally, we examined stability of JMV 1843 in mouse blood serum. A single subcutaneous injection of JMV 1843 (0.01-10 mg/kg) increased food intake in fed mice in a dosedependent manner, up to 5-times relative to the saline-treated group (ED50=1.94 mg/kg at 250 min). JMV 1843 was stable in mouse serum in vitro for 24 h, but was mostly eliminated from mouse blood after 2 h in vivo. Ten days of treatment with JMV 1843 (subcutaneous administration, 10 or 20 mg/kg/day) significantly increased food intake, body weight and mRNA expression of the orexigenic neuropeptide Y and agouti-related peptide in the medial basal hypothalamus and decreased the expression of uncoupling protein 1 in brown adipose tissue. Our data suggest that JMV 1843 could have possible future uses in the treatment of cachexia.
- Subjects
GHRELIN receptors; CACHEXIA treatment; BODY weight; NEUROPEPTIDES; GENE expression; MESSENGER RNA; LABORATORY mice
- Publication
Physiological Research, 2013, Vol 62, Issue 4, p435
- ISSN
0862-8408
- Publication type
Article
- DOI
10.33549/physiolres.932488