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- Title
Abnormal expression of Pygopus 2 correlates with a malignant phenotype in human lung cancer.
- Authors
Yang Liu; Qian-Ze Dong; Si Wang; Chang-Qing Fang; Yuan Miao; Liang Wang; Ming-Zhu Li; En-Hua Wang; Liu, Yang; Dong, Qian-Ze; Wang, Si; Fang, Chang-Qing; Miao, Yuan; Wang, Liang; Li, Ming-Zhu; Wang, En-Hua
- Abstract
<bold>Background: </bold>Pygopus 2 (Pygo2) is a Pygo family member and an important component of the Wnt signaling transcriptional complex. Despite this data, no clinical studies investigating Pygo2 expression in lung cancer have yet been reported.<bold>Methods: </bold>In the present study, the expression patterns of Pygo2 were evaluated by immunochemistry in 168 patients with non-small cell lung cancer (NSCLC). We used small interfering RNA (siRNA) to specifically silence Pygo2, and investigated its effect on cell growth by an 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and flow cytometry analysis in human lung cancer cell lines.<bold>Results: </bold>Immunohistochemical analysis showed low expression of Pygo2 in normal lung tissues and increased nuclear expression in lung cancer tissues, either with or without perinuclear expression. Abnormal Pygo2 expression was associated with poor differentiation and a high Tumor (T), Node (N) and Metastases (M) stage in NSCLC patients, and correlated with poor prognosis. Using MTT assay we observed that Pygo2 downregulation inhibited cell proliferation; in addition, flow cytometry analysis showed that Pygo2 knockdown induced apoptosis and increased numbers of G1-phase cells and a reduction in S-phase cells.<bold>Conclusions: </bold>We therefore conclude that abnormal Pygo2 protein expression may be a marker for advanced NSCLC. Furthermore, Pygo2 knockdown suppresses cell growth.
- Subjects
LUNG cancer; PHENOTYPES; GENE expression; SMALL interfering RNA; IMMUNOHISTOCHEMISTRY; CELL proliferation; TUMOR growth; FLOW cytometry; REVERSE transcriptase polymerase chain reaction; RESEARCH; WESTERN immunoblotting; RESEARCH methodology; SIGNAL peptides; LUNG tumors; RNA; MEDICAL cooperation; EVALUATION research; COMPARATIVE studies; GENETIC techniques; POLYMERASE chain reaction
- Publication
BMC Cancer, 2013, Vol 13, Issue 1, p1
- ISSN
1471-2407
- Publication type
journal article
- DOI
10.1186/1471-2407-13-346