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- Title
The CA domain of the respiratory complex I is required for normal embryogenesis in Arabidopsis thaliana.
- Authors
Córdoba, Juan Pablo; Marchetti, Fernanda; Soto, Débora; Martin, María Victoria; Pagnussat, Gabriela Carolina; Zabaleta, Eduardo
- Abstract
The NADH-ubiquinone oxidoreductase [complex I (CI), EC 1.6.5.3] of the mitochondrial respiratory chain is the principal entry point of electrons, and vital in maintaining metabolism and the redox balance. In a variety of eukaryotic organisms, except animal and fungi (Opisthokonta), it contains an extra domain composed of putative gamma carbonic anhydrases subunits, named the CA domain, which was proposed to be essential for complex I assembly. There are two kinds of carbonic anhydrase subunits: CAs (of which there are three) and carbonic anhydrase-like proteins (CALs) (of which there are two). In plants, the CA domain has been linked to photorespiration. In this work, we report that Arabidopsis mutant plants affected in two specific CA subunits show a lethal phenotype. Double homozygous knockouts ca1ca2 embryos show a significant developmental delay compared to the non-homozygous embryos, which show a wild-type (WT) phenotype in the same silique. Mutant embryos show impaired mitochondrial membrane potential and mitochondrial reactive oxygen species (ROS) accumulation. The characteristic embryo greening does not take place and fewer but larger oil bodies are present. Although seeds look dark brown and wrinkled, they are able to germinate 12 d later than WT seeds. However, they die immediately, most likely due to oxidative stress. Since the CA domain is required for complex I biogenesis, it is predicted that in ca1ca2 mutants no complex I could be formed, triggering the lethal phenotype. The in vivo composition of a functional CA domain is proposed.
- Subjects
PLANT embryology; CARBONIC anhydrase; MEMBRANE potential; REACTIVE oxygen species; RESPIRATION in plants; PLANTS
- Publication
Journal of Experimental Botany, 2016, Vol 67, Issue 5, p1589
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erv556