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- Title
A Tunable Nanoplatform of Nanogold Functionalised with Angiogenin Peptides for Anti-Angiogenic Therapy of Brain Tumours.
- Authors
Naletova, Irina; Cucci, Lorena Maria; D'Angeli, Floriana; Anfuso, Carmelina Daniela; Magrì, Antonio; La Mendola, Diego; Lupo, Gabriella; Satriano, Cristina
- Abstract
Angiogenin (ANG), an endogenous protein that plays a key role in cell growth and survival, has been scrutinised here as promising nanomedicine tool for the modulation of pro-/anti-angiogenic processes in brain cancer therapy. Specifically, peptide fragments from the putative cell membrane binding domain (residues 60–68) of the protein were used in this study to obtain peptide-functionalised spherical gold nanoparticles (AuNPs) of about 10 nm and 30 nm in optical and hydrodynamic size, respectively. Different hybrid biointerfaces were fabricated by peptide physical adsorption (Ang60–68) or chemisorption (the cysteine analogous Ang60–68Cys) at the metal nanoparticle surface, and cellular assays were performed in the comparison with ANG-functionalised AuNPs. Cellular treatments were performed both in basal and in copper-supplemented cell culture medium, to scrutinise the synergic effect of the metal, which is another known angiogenic factor. Two brain cell lines were investigated in parallel, namely tumour glioblastoma (A172) and neuron-like differentiated neuroblastoma (d-SH-SY5Y). Results on cell viability/proliferation, cytoskeleton actin, angiogenin translocation and vascular endothelial growth factor (VEGF) release pointed to the promising potentialities of the developed systems as anti-angiogenic tunable nanoplaftforms in cancer cells treatment.
- Subjects
BIOLOGICAL assay; BRAIN tumors; CARRIER proteins; CELL lines; CELL physiology; COPPER; CULTURE media (Biology); GLIOMAS; GOLD compounds; NANOPARTICLES; PEPTIDES; VASCULAR endothelial growth factors
- Publication
Cancers, 2019, Vol 11, Issue 9, p1322
- ISSN
2072-6694
- Publication type
Article
- DOI
10.3390/cancers11091322