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- Title
Performance evaluation of a novel reticulocyte identification method that uses metachromatic nucleic acid staining based on a crossover analysis of emission DNA/RNA light (RNP Determination™) in hematology analyzer Celltac G+.
- Authors
Kaori Yahagi; Tomoko Arai; Hisako Katagiri; Yoko Yatabe; Hiromitsu Yokota; Yutaka Nagai; Takayuki Mitsuhashi; Masatoshi Wakui; Mitsuru Murata
- Abstract
Introduction: Assessing the percentage of reticulocytes (%Retic) is useful for diagnosing and treating blood diseases that present with anaemia. The Celltac G+™ hematology analyzer (HA) uses a novel reticulocyte identification method that involves metachromatic nucleic acid staining with acridine orange and crossover analysis of emission light of DNA/RNA (determination of red cells, nucleic acid-containing cells, and platelets, RNP Determination™). The red and green fluorescence generated by stained singlestranded RNA and double-stranded DNA express immaturity and morphological abnormality of erythrocytes by detecting erythrocyte RNA and DNA content. Methods: The basic performance of the test automated analyzer (TAA) Celltac G+ was evaluated and compared with the flow cytometry reference method and the comparative automated analyzer (CAA) XN-1000/2000™. In addition, its precision, limit of quantity (LoQ), linearity, analytical measurement interval (AMI), accuracy, and comparability and the effects of interfering substances were evaluated. Results: Evaluation of %Retic by the TAA demonstrated good precision and linearity. The AMI was confirmed from 0.02 to 8.23, and the LoQ in %Retic as the coefficient of variation within an 11% limit (SD, within a 0.01 limit) was 0.14. TAA correlated well with the reference method and routine HA (CAA). Some deviations were found between TAA and CAA in DNA measurements of erythrocytes from abnormal samples. Conclusion: Celltac G+ uses a novel measurement principle and can assess erythrocyte immaturity independent of DNA contents. It represents a new HA that provides novel, useful information on immaturity and morphological abnormality of erythrocytes.
- Subjects
FLOW cytometry; DNA; RETICULOCYTES; STAINS &; staining (Microscopy); RESEARCH evaluation; HEMATOLOGY; AUTOANALYZERS; BLOOD platelets; RNA; COMPARATIVE studies; DESCRIPTIVE statistics; NUCLEIC acids
- Publication
International Journal of Laboratory Hematology, 2022, Vol 44, Issue 6, p1050
- ISSN
1751-5521
- Publication type
Article
- DOI
10.1111/ijlh.13947