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- Title
Porcine jejunal alkaline phosphatase gene expression is quadratically changed during the postnatal growth examined by the quantitative real-time RT PCR.
- Authors
Tiejun Li; Chengbo Yang; Lackeyram, Dale; Yulong Yin; Fan, Ming Z.
- Abstract
Intestinal alkaline phosphatase (AP) is responsible for hydrolyzing phosphoric ester bonds of organic compounds and likely plays a role in intestinal transcellular nutrient transport. This study was conducted to examine porcine jejunal AP gene expression (mRNA) during the postnatal growth. A total of 36 littermate purebred Yorkshire gilts were divided into six groups at the ages old 1, 4, 6, 12, 20 (suckling) and 28 (1 wk post-weaning), respectively. Relative jejunal AP mRNA abundance was measured by the real time RT-PCR analysis (SmartCycler) using the SYBR Green-I detection kit with glyceraldehydes-3 -phosphate dehydrogenase (GAPDH) as the housekeeping gene. Orthogonal polynomial contrast analyses of the data showed a quadratic pattern (P=0.013) of the porcine jejunal AP mRNA expression (LSMEAN, 1.43, 2.50, 2.90, 4.32, 3.27 and 0.38, respectively, with a pooled SD=2.76) at the ages of d 1, 4, 6, 12 and 20, and 28. These results suggest that porcine jejunal alkaline phosphatase gene expression was increased from birth to the peak at 12-d suckling and dramatically decreased during the weaning between d 21 and 28.
- Subjects
ALKALINE phosphatase; GENE expression; MESSENGER RNA; LABORATORY swine; GROWTH; INTESTINES; PHOSPHATASES
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1076
- ISSN
0892-6638
- Publication type
Article