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- Title
Production of germline transgenic chickens expressing enhanced green fluorescent protein using a MoMLV-based retrovirus vector.
- Authors
Bon Chul Koo; Mo Sun Kwon; Bok Ryul Choi; Jin-Hoi Kim; Seong-Keum Cho; Sea Hwan Sohn; Eun Jung Cho; Hoon Taek Lee; Wonkyung Chang; Iksoo Jeon; Jin-Ki Park; Jae Bok Park; Teoan Kim
- Abstract
The Moloney murine leukemia virus (MoMLV) -based retrovirus vector system has been used most often in gene transfer work, but has been known to cause silencing of the imported gene in transgenic animals. In the present study, using a MoMLV-based retrovirus vector, we successfully generated a new transgenic chicken line expressing high levels of enhanced green fluorescent protein (eGFP). The level of eGFP expression was conserved after germline transmission and as much as 100 µg of eGFP could be detected per 1 mg of tissue protein. DNA sequencing showed that the transgene had been integrated at chromosome 26 of the G1 and G2 generation transgenic chickens. Owing to the stable integration of the transgene, it is now feasible to produce G3 generation of homozygous eGFP transgenic chickens that will provide 100% transgenic eggs. These results will help establish a useful transgenic chicken model system for studies of embryonic development mid for efficient production of transgenic chickens as bioreactors.
- Subjects
MOUSE leukemia viruses; MOUSE leukemia; RETROVIRUSES; GENETIC transformation; GREEN fluorescent protein; BIOREACTORS
- Publication
FASEB Journal, 2006, Vol 20, Issue 13, p2251
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.06-5866com