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- Title
Matrix and Steiner-triple-system smart pooling assays for high-performance transcription regulatory network mapping.
- Authors
Vermeirssen, Vanessa; Deplancke, Bart; Barrasa, M Inmaculada; Reece-Hoyes, John S; Arda, H. Efsun; Grove, Christian A.; Martinez, Natalia J.; Sequerra, Reynaldo; Doucette-Stamm, Lynn; Brent, Michael R.; Walhout, Albertha J. M.
- Abstract
Yeast one-hybrid (Y1H) assays provide a gene-centered method for the identification of interactions between gene promoters and regulatory transcription factors (TFs). To date, Y1H assays have involved library screens that are relatively expensive and laborious. We present two Y1H strategies that allow immediate prey identification: matrix assays that use an array of 755 individual Caenorhabditis elegans TFs, and smart-pool assays that use TF multiplexing. Both strategies simplify the Y1H pipeline and reduce the cost of protein-DNA interaction identification. We used a Steiner triple system (STS) to create smart pools of 4–25 TFs. Notably, we uniplexed a small number of highly connected TFs to allow efficient assay deconvolution. Both strategies outperform library screens in terms of coverage, confidence and throughput. These versatile strategies can be adapted both to TFs in other systems and, likely, to other biomolecules and assays as well.
- Subjects
TRANSCRIPTION factors; CAENORHABDITIS elegans; MULTIPLEXING; DNA; BIOMOLECULES; GENE expression
- Publication
Nature Methods, 2007, Vol 4, Issue 8, p659
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth1063