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- Title
Interaction of the K<sup>+</sup> channel KcsA with membrane phospholipids as studied by ESI mass spectrometry
- Authors
Demmers, Jeroen A.A.; van Dalen, Annemieke; de Kruijff, Ben; Heck, Albert J.R.; Killian, J. Antoinette
- Abstract
In this study we have used electrospray ionization mass spectrometry (ESI-MS) to investigate interactions between the bacterial K+ channel KcsA and membrane phospholipids. KcsA was reconstituted into lipid vesicles of variable lipid composition. These vesicles were directly analyzed by ESI-MS or mixed with trifluoroethanol (TFE) before analysis. In the resulting mass spectra, non-covalent complexes of KcsA and phospholipids were observed with an interesting lipid specificity. The anionic phosphatidylglycerol (PG), and, to a lesser extent, the zwitterionic phosphatidylethanolamine (PE), which both are abundant bacterial lipids, were found to preferentially associate with KcsA as compared to the zwitterionic phosphatidylcholine (PC). These preferred interactions may reflect the differences in affinity of these phospholipids for KcsA in the membrane.
- Subjects
ELECTROSPRAY ionization mass spectrometry; PHOSPHOLIPIDS
- Publication
FEBS Letters, 2003, Vol 541, Issue 1-3, p28
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/S0014-5793(03)00282-5