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- Title
Enhanced survival of ultraviolet-irradiated herpes simplex virus in carcinogen-pretreated cells.
- Authors
LYTLE, C. DAVID; COPPEY, JACQUES; TAYLOR, WILLIAM D.
- Abstract
WHEN CV-1 monkey kidney host cells are exposed to low doses of ultraviolet (UV) or X-ray radiation, or to photodynamic treatment before infection with herpes simplex virus type 1 (HSV), the survival of UV-irradiated virus is increased1-3. This increase is more pronounced if the time interval between cell UV-irradiation and virus infection is increased by a few days4. A similarly increased survival of UV-damaged phage λ is observed in bacteria damaged by UV, X rays, thymine starvation, exposure to mitomycin C, or thermal shift of tif-1 mutants5. Moreover, it has been shown that enhanced survival of UV-damaged phage λ occurred in bacteria previously treated with enzymatically activated aflatoxin B1, a potent hepatocarcinogen6. The purpose of this study was to determine whether treatment of CV-1 cells with carcinogens would result in enhanced survival of UV-inactivated HSV. We chose for this purpose (1) aflatoxin B1 because, after treatment with this activated mycotoxin, human fibroblast cells exhibit a UV-like repair replication, the extent of which is at the very most 20% of that observed after UV irradiation7, and (2) acetylamino-fluorene (AAF) and its derivatives, n-acetoxy-2-AAF and n-hydroxy-2-AAF, as these derivatives are known to be active intermediates in the carcinogenic activity of the parental compound8, and because, in n-acetoxy-AAF-treated human cells, there is a UV-like repair response9. Virus reactivation was assayed in the same manner as that used in previous investigations of radiation-enhanced reactivation1-4.
- Publication
Nature, 1978, Vol 272, Issue 5648, p60
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/272060a0