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- Title
Polymer-based alternative method to extract bromelain from pineapple peel waste.
- Authors
Lencastre Novaes, Letícia Celia; Carvalho Santos Ebinuma, Valéria; Mazzola, Priscila Gava; Júnior, Adalberto Pessoa
- Abstract
Bromelain is a mixture of proteolytic enzymes present in all tissues of the pineapple ( Ananas comosus Merr.), and it is known for its clinical therapeutic applications, food processing, and as a dietary supplement. The use of pineapple waste for bromelain extraction is interesting from both an environmental and a commercial point of view, because the protease has relevant clinical potential. We aimed to study the optimization of bromelain extraction from pineapple waste, using the aqueous two-phase system formed by polyethylene glycol ( PEG) and poly(acrylic acid). In this work, bromelain partitioned preferentially to the top/ PEG-rich phase and, in the best condition, achieved a yield of 335.27% with a purification factor of 25.78. The statistical analysis showed that all variables analyzed were significant to the process.
- Subjects
POLYMERS; PLANT extracts; BROMELIN; PINEAPPLE; FRUIT skins; PROTEOLYTIC enzymes
- Publication
Biotechnology & Applied Biochemistry, 2013, Vol 60, Issue 5, p527
- ISSN
0885-4513
- Publication type
Article
- DOI
10.1002/bab.1121