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- Title
Optical and topographic characteristics of silver films deposited from a colloidal solution on polyelectrolytes for IgG-FITC fluorescence analysis.
- Authors
Kulakovich, Olga; Scherbovich, Anastasia; Koktysh, Irina; Melnikova, Yanina; Ramanenka, Andrei; Gaponenko, Sergey; Maskevich, Sergey
- Abstract
The optimization of the fluorescence enhancement factor of the IgG-FITC conjugate as an immunofluorescent marker, depending on the optical and topographic parameters of the colloidal silver film on the surface of a polystyrene plate for immunoassay, was carried out for the first time, and the factors effecting the enhancement were identified. By means of time-resolved spectroscopy as well as by detection the relative concentrations of IgG-FITC adsorbed on the solid phase with enzyme-linked immunoassay, it was shown that fluorescence enhancement in the presence of silver nanoparticles is a resonance process associated with plasmon effects. The most important parameters correlating with the fluorescence enhancement factor are the optical density value at the wavelength of excitation and emission of the fluorophore. The maximum enhancement factor of 10.2 times was obtained for Ag films with the highest optical density.
- Subjects
FLUORIMETRY; COLLOIDAL silver; OPACITY (Optics); POLYELECTROLYTES; SILVER nanoparticles; TIME-resolved spectroscopy; SILVER ions; RAMAN scattering
- Publication
Zeitschrift für Physikalische Chemie, 2022, Vol 236, Issue 11/12, p1603
- ISSN
0942-9352
- Publication type
Article
- DOI
10.1515/zpch-2021-3110