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- Title
Lipolytic Activity of a Carboxylesterase from Bumblebee (Bombus ignitus) Venom.
- Authors
Deng, Yijie; Kim, Bo Yeon; Lee, Kyeong Yong; Yoon, Hyung Joo; Wan, Hu; Li, Jianhong; Lee, Kwang Sik; Jin, Byung Rae
- Abstract
Bee venom is a complex mixture composed of peptides, proteins with enzymatic properties, and low-molecular-weight compounds. Although the carboxylesterase in bee venom has been identified as an allergen, the enzyme's role as a venom component has not been previously elucidated. Here, we show the lipolytic activity of a bumblebee (Bombus ignitus) venom carboxylesterase (BivCaE). The presence of BivCaE in the venom secreted by B. ignitus worker bees was confirmed using an anti-BivCaE antibody raised against a recombinant BivCaE protein produced in baculovirus-infected insect cells. The enzymatic activity of the recombinant BivCaE protein was optimal at 40 °C and pH 8.5. Recombinant BivCaE protein degrades triglycerides and exhibits high lipolytic activity toward long-chain triglycerides, defining the role of BivCaE as a lipolytic agent. Bee venom phospholipase A2 binds to mammalian cells and induces apoptosis, whereas BivCaE does not affect mammalian cells. Collectively, our data demonstrate that BivCaE functions as a lipolytic agent in bee venom, suggesting that BivCaE will be involved in distributing the venom via degradation of blood triglycerides.
- Subjects
BEE venom; BUMBLEBEES; VENOM; RECOMBINANT proteins; DERMATOPHAGOIDES; ALLERGENS; BEES
- Publication
Toxins, 2021, Vol 13, Issue 4, p239
- ISSN
2072-6651
- Publication type
Article
- DOI
10.3390/toxins13040239