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- Title
MicroRNA-101 inhibits growth and metastasis of human ovarian cancer cells by targeting PI3K/AKT.
- Authors
Min Wei; Hongjuan Jin; ShuLi Yang; Zhuo Li; Xinlei Wang; LiXiang Li; Yan Jia; ManHua Cui; Wei, Min; Jin, Hongjuan; Yang, ShuLi; Li, Zhuo; Wang, Xinlei; Li, LiXiang; Jia, Yan; Cui, ManHua
- Abstract
<bold>Introduction: </bold>Ovarian cancer is the most frequent cause of gynecological cancer related mortality in woman. This study was designed to investigate the role and therapeutic potential of miRNA-101 in ovarian cancer.<bold>Material and Methods: </bold>Expression analysis was carried out by real-time quantitative polymerase chain reaction. Transfections were performed with the help of Lipofectamine 2000 reagent. AO/EB and annexin V/PI staining was used to detect apoptosis and flow cytometry was used for cell cycle analysis. Western blotting was employed for cell cycle analysis.<bold>Results: </bold>It was found that miRNA-101 was significantly down-regulated in ovarian cancer cells. The over-expression of miRNA-101 causes a significant decrease in the viability of ovarian cancer cells via the initiation of apoptosis and sub-G1 arrest of OVACAR-3 cells. It was indicated that PTEN was the potential target of miRNA-101 in OVACAR-3 cells. There was 4.5-fold up-regulation of PTEN expression in ovarian cancer cell lines and the over-expression of miRNA-101 in OVACAR-3 cells resulted in the down-regulation of PTEN expression. The inhibition of PTEN in the OVACAR-3 cells arrested the proliferation of these cells. The over-expression of miRNA-101 causes significant down-regulation in PI3K and AKT expression of OVACAR-3 cells.<bold>Conclusions: </bold>It can be concluded that miRNA-101 acts as a tumor suppressor which may be beneficial in the treatment of ovarian cancer.
- Subjects
OVARIAN cancer; CANCER cell growth; CANCER cells; HUMAN growth; CELL analysis; CELL cycle; POLYMERASE chain reaction
- Publication
Archives of Medical Science, 2021, Vol 17, Issue 1, p127
- ISSN
1734-1922
- Publication type
journal article
- DOI
10.5114/aoms.2019.85404