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- Title
Time-tagged ticker tapes for intracellular recordings.
- Authors
Lin, Dingchang; Li, Xiuyuan; Moult, Eric; Park, Pojeong; Tang, Benjamin; Shen, Hao; Grimm, Jonathan B.; Falco, Natalie; Jia, Bill Z.; Baker, David; Lavis, Luke D.; Cohen, Adam E.
- Abstract
Recording transcriptional histories of a cell would enable deeper understanding of cellular developmental trajectories and responses to external perturbations. Here we describe an engineered protein fiber that incorporates diverse fluorescent marks during its growth to store a ticker tape-like history. An embedded HaloTag reporter incorporates user-supplied dyes, leading to colored stripes that map the growth of each individual fiber to wall clock time. A co-expressed eGFP tag driven by a promoter of interest records a history of transcriptional activation. High-resolution multi-spectral imaging on fixed samples reads the cellular histories, and interpolation of eGFP marks relative to HaloTag timestamps provides accurate absolute timing. We demonstrate recordings of doxycycline-induced transcription in HEK cells and cFos promoter activation in cultured neurons, with a single-cell absolute accuracy of 30–40 minutes over a 12-hour recording. The protein-based ticker tape design we present here could be generalized to achieve massively parallel single-cell recordings of diverse physiological modalities. Reporter gene expression history in cells is recorded with engineered fluorescent protein fibers.
- Publication
Nature Biotechnology, 2023, Vol 41, Issue 5, p631
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/s41587-022-01524-7