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- Title
Prognostic significance of DNA ploidy in adenocarcinoma of the pancreas. A flow cytometric study of paraffin-embedded specimens.
- Authors
Porschen, Rainer; Remy, Udo; Bevers, Gerd; Schauseil, Stephan; Hengels, Klaus-Jürgen; Borchard, Franz; Porschen, R; Remy, U; Bevers, G; Schauseil, S; Hengels, K J; Borchard, F
- Abstract
<bold>Background: </bold>The prognostic significance of tumor DNA ploidy in patients with cancer of the pancreas has not been defined because conflicting results have been reported.<bold>Methods: </bold>DNA content was measured in 56 ductal adenocarcinomas of the pancreas. DNA ploidy status was evaluated by flow cytometry in nuclei isolated from paraffin-embedded tumor tissues.<bold>Results: </bold>An abnormal DNA stemline was observed in 27 (48%) patients. The percentage of aneuploid tumors was significantly increased in tumors classified as Stage III/IV (53%) compared with those classified as Stage I (22%). A borderline significant association existed between DNA ploidy and radicality of surgery (P = 0.08). The median survival of patients with diploid carcinomas was 6.9 months (standard error, +/- 0.9) in comparison to 4.5 +/- 1.2 months for patients with aneuploid tumors (P = 0.013 by generalized Wilcoxon test; P = 0.023 by generalized Savage test). Although a selection bias cannot be excluded, survival of patients with a radical resection was longer than that of patients with a nonradical resection (P = 0.0008 and P = 0.0085, respectively). In addition, presence of distant metastasis (P = 0.0006 [Wilcoxon test] and P = 0.033 [Savage test]) could be identified as a prognostic factor. In a Cox regression model, results of surgery and DNA ploidy were independent prognostic variables.<bold>Conclusions: </bold>Because DNA ploidy has a significant impact on prognosis in pancreatic cancer, it should be used as a variable for stratified randomization of patients in therapeutic trials.
- Subjects
PANCREATIC tumors; FLOW cytometry; ADENOCARCINOMA; STATISTICS; SURVIVAL; DNA; ANEUPLOIDY; LIFE expectancy; PROGNOSIS; CELL physiology; TUMOR classification; GENES; HISTOLOGICAL techniques
- Publication
Cancer (0008543X), 1993, Vol 71, Issue 12, p3846
- ISSN
0008-543X
- Publication type
journal article
- DOI
10.1002/1097-0142(19930615)71:12<3846::AID-CNCR2820711209>3.0.CO;2-I