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- Title
Characterization of T-cell receptor β chain mRNA expression in IFN-α-responsive chronic myelogenous leukaemia patients.
- Authors
Shimomura, Taizo; Fujii, Shin-ichiro; Ezaki, Ichiko; Osato, Motomi; Fujimoto, Koji; Takatsuki, Kiyoshi; Yamamoto, Kazuhiko; Kawakita, Makoto
- Abstract
Interferon-α (IFN-α) has shown promise in the treatment of chronic phase of chronic myelogenous leukaemia (CML). IFN-α has also been found to indirectly up-regulate the expression of major histocompatibility (MHC) antigens, and to directly increase the activity of lymphocytes against tumour cells. To elucidate whether IFN-α induces anti-leukaemic activity of the autologous T cells in CML patients, we analysed the accumulation of T-cell receptor (TCR) in each Vβ family using the reverse transcriptase-polymerase chain reaction (RT-PCR) followed by single-strand conformation (SSCP) analysis. We found the predominant expression of the Vβ 10, 12, and 14 families in the peripheral blood (PB) of CML patients, in contrast to healthy donors. Especially, in IFN-α-responsive patients, we observed an enhancement of the accumulation of the Vβ 9 and 20 families, suggesting that T cells enhanced by IFN-α may react with a discrete set of antigens on the surface of malignant cells. These findings may demonstrate that CML cells possess the heterogenous antigens and the clonal expansion of Vβ 9+ and Vβ 20+ T cells may be a prognostic indicator of the immune responsiveness to IFN-α.
- Subjects
INTERFERONS; T cell receptors; MYELOID leukemia
- Publication
British Journal of Haematology, 1999, Vol 105, Issue 1, p173
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1111/j.1365-2141.1999.01283.x