We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
ABNOH‐Linked Nucleotides and DNA for Bioconjugation and Cross‐linking with Tryptophan‐Containing Peptides and Proteins.
- Authors
Spampinato, Ambra; Leone, Denise‐Liu'; Pohl, Radek; Tarábek, Ján; Šoltysová, Markéta; Polák, Marek; Kádek, Alan; Sýkorová, Veronika; Řezáčová, Pavlína; Hocek, Michal
- Abstract
Reactive N‐hydroxy‐9‐azabicyclo[3.3.1]nonane (ABNOH) linked 2′‐deoxyuridine 5′‐O‐mono‐ and triphosphates were synthesized through a CuAAC reaction of ABNOH‐PEG4‐N3 with 5‐ethynyl‐dUMP or ‐dUTP. The modified triphosphate was used as substrate for enzymatic synthesis of modified DNA probes with KOD XL DNA polymerase. The keto‐ABNO radical reacted with tryptophan (Trp) and Trp‐containing peptides to form a stable tricyclic fused hexahydropyrrolo‐indole conjugates. Similarly modified ABNOH‐linked nucleotides reacted with Trp‐containing peptides to form a stable conjugate in the presence but surprisingly even in the absence of NaNO2 (presumably through activation by O2). The reactive ABNOH‐modified DNA probe was used for bioconjugations and crosslinking with Trp‐containing peptides or proteins.
- Subjects
DNA probes; DNA polymerases; DNA synthesis; BIOCHEMICAL substrates; PEPTIDES
- Publication
Chemistry - A European Journal, 2024, Vol 30, Issue 49, p1
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.202402151