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- Title
Ionizing Radiation Potentiates the Induction of Nitric Oxide Synthase by Interferon-γ and/or Lipopolysaccharide in Murine Macrophage Cell Lines: Role of Tumor Necrosis Factor-α.
- Authors
MCKINNEY, LESLIE C.; AQUILLA, ELIZABETH M.; COFFIN, DEBORAH; WINK, DAVID A.; VODOVOTZ, YORAM
- Abstract
A bstract: Macrophages respond to infection or injury by changing from a 'resting' cellular phenotype to an 'activated' state defined by the expression of various cytotoxic effector functions. Regulation of the transition from a resting to an activated state is effected by cytokine and/or pathogenic signals. Some signals do not directly induce activation, but instead 'prime' the macrophage to respond more vigorously to a second signal. One example of this priming phenomenon involves induction of nitric oxide (NO) synthesis by the enzyme nitric oxide synthase (NOS2). Our experiments indicate that low doses (1-5 Gy) of ionizing radiation can enhance the induction of enzymatically active NOS2 by IFN-γ or LPS in J774.1 and RAW264.7 murine macrophage cell lines. Radiation alone did not produce this induction, rather, it was effective as a priming signal; cells exposed to radiation produced more NO when a second signal, either IFN-γ or LPS, was applied 24 h later.
- Publication
Annals of the New York Academy of Sciences, 2000, Vol 899, Issue 1, p61
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1111/j.1749-6632.2000.tb06176.x