We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Transcriptome alterations in HepG2 cells induced by shRNA knockdown and overexpression of TMEM2 gene.
- Authors
Jia, Xiuhua; Mo, Zhishuo; Zhao, Qiyi; Bao, Tiancheng; Xu, Wexiong; Gao, Zhiliang; Peng, Liang; Zhu, Xiang
- Abstract
Transmembrane 2 (TMEM2) gene inhibits chronic hepatitis-B virus (HBV) infection, while the underlying molecular mechanisms remain unknown. Transcriptome alterations in HepG2 cells following TMEM2 overexpression or silencing by shRNA were analyzed by next-generation sequencing. Both overexpression and knockdown of the TMEM2 gene caused wide-spread changes in gene expression in HepG2 cells. Differentially expressed genes caused by altered TMEM2 gene expression were associated with multiple biological processes linked with viral infection and various signaling pathways. KEGG analysis revealed that many of the differentially expressed genes were enriched in the PI3K/AKT signaling pathway. Moreover, we show that genes related to the PI3K/AKT signaling pathway, such as SYK, FLT4, AKT3, FLT1, and IL6, are biological targets regulated by TMEM2 in HepG2 cells. This is the first transcriptome-wide study in which TMEM2-regulated genes in HepG2 cells have been screened. Our findings elucidate the molecular events associated with TMEM2-mediated hepatocyte pathogenesis in chronic HBV infection. A large-scale analysis of gene expression profile alterations in HepG2 cells induced by TMEM2 knockdown and overexpression by transcriptome sequencing.
- Subjects
GENETIC overexpression; GENE expression profiling; GENE expression; PATHOLOGY; VIRUS diseases
- Publication
Bioscience, Biotechnology & Biochemistry, 2020, Vol 84, Issue 8, p1576
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2020.1756733