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- Title
Phenylethanoid glycosides from Paraboea martinii protect rat pheochromocytoma (PC12) cells from hydrogen peroxide-induced cell injury.
- Authors
Gong, Xue; Xu, Yan; Ren, Kai; Bai, Xiaorong; Zhang, Chunhong; Li, Minhui
- Abstract
In this study, we isolated eight phenylethanoid glycosides from Paraboea martinii for the first time, and evaluated the mechanism underlying their neuroprotective effects against H2O2-induced injury in PC12 cells. The MTS method was utilized to screen the phenylethanoid glycosides for protective ability. Next, qRT-PCR and western blotting analysis were used to detect the transcription levels of HO-1 and GCLC, which are regulated by Nrf2. The inhibitor ZnPP was used to analyze the involvement of Nrf2 in HO-1 expression. Analyses showed that caleolarioside B, paraboside B, and paraboside II also upregulated the expression of HO-1, but showed no obvious effect on GCLC. Pretreatment with ZnPP significantly reduced the neuroprotective effects. Thus, phenylethanoid glycosides isolated from P. martinii protected PC12 cells from H2O2-induced damage by upregulating HO-1. The results provided evidence that P. martinii might be a potential therapeutic agent for the treatment of neurodegenerative diseases. Phenylethanoid glycosides was isolated from P. martinii could protect PC12 cells from.
- Subjects
PHEOCHROMOCYTOMA; GLYCOSIDES; WESTERN immunoblotting; NEURODEGENERATION
- Publication
Bioscience, Biotechnology & Biochemistry, 2019, Vol 83, Issue 12, p2202
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2019.1654359