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- Title
Determination of low molecular weight organic acids in soil, plants, and water by capillary zone electrophoresis.
- Authors
Ying-Hui Li; Bi-Xia Huang; Xiao-Quan Shan
- Abstract
Determination of low molecular weight organic acids in soils and plants by capillary zone electrophoresis was accomplished using a phthalate buffer and indirect UV detection mode. The influence of some crucial parameters, such as pH, buffer concentration and surfactant were investigated. A good separation of seven organic acids was achieved within 5 min using an electrolyte containing 15 mmol L-1 potassium hydrogen phthalate, 0.5 mmol L-1 myristyltrimethylammonium bromide (MTAB), and 5% methanol (MeOH) (v/v) at pH 5.60, separation voltage -20 kV, and temperature 25 °C. The relative standard deviation (n=5) of the method was found to be in range 0.18–0.56% for migration time and 3.2–4.8% for peak area. The limit of detection ranged between 0.5 µmol L-1 to 6 µmol L-1 at a signal-to-noise ratio of 3. The recovery of standard organic acids added to real samples ranged from 87 to 119%. This method was simple, rapid and reproducible, and could be applied to the simultaneous determination of organic acids in environmental samples.
- Subjects
MOLECULAR weights; ORGANIC acids; CAPILLARY electrophoresis; ZONE electrophoresis; HYDROGEN-ion concentration; PLANTS; SOILS
- Publication
Analytical & Bioanalytical Chemistry, 2003, Vol 375, Issue 6, p775
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-003-1789-1