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- Title
Discriminative Detection of Biothiols by Electron Paramagnetic Resonance Spectroscopy using a Methanethiosulfonate Trityl Probe.
- Authors
Tan, Xiaoli; Ji, Kaiyun; Wang, Xing; Yao, Ru; Han, Guifang; Villamena, Frederick A.; Zweier, Jay L.; Song, Yuguang; Rockenbauer, Antal; Liu, Yangping
- Abstract
Biothiols, such as glutathione (GSH), homocysteine (Hcy), and cysteine (Cys), coexist in biological systems with diverse biological roles. Thus, analytical techniques that can detect, quantify, and distinguish between multiple biothiols are desirable but challenging. Herein, we demonstrate the simultaneous detection and quantitation of multiple biothiols, including up to three different biothiols in a single sample, using electron paramagnetic resonance (EPR) spectroscopy and a trityl‐radical‐based probe (MTST). We term this technique EPR thiol‐trapping. MTST could trap thiols through its methanethiosulfonate group to form the corresponding disulfide conjugate with an EPR spectrum characteristic of the trapped thiol. MTST was used to investigate effects of l‐buthionine sulfoximine (BSO) and pyrrolidine dithiocarbamate (PDTC) on the efflux of GSH and Cys from HepG2 cells.
- Subjects
ELECTRON paramagnetic resonance spectroscopy; ELECTRON paramagnetic resonance; ELECTRON detection; BIOLOGICAL systems
- Publication
Angewandte Chemie, 2020, Vol 132, Issue 2, p938
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201912832