We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Surface Immobilization of Redox‐Labile Fluorescent Probes: Enabling Single‐Cell Co‐Profiling of Aerobic Glycolysis and Oncogenic Protein Signaling Activities.
- Authors
Li, Zhonghan; Cheng, Hanjun; Shao, Shiqun; Lu, Xiang; Mo, Li; Tsang, Jonathan; Zeng, Pu; Guo, Zhili; Wang, Siwen; Nathanson, David A.; Heath, James R.; Wei, Wei; Xue, Min
- Abstract
Abstract: An analytical method is described for profiling lactate production in single cells via the use of coupled enzyme reactions on surface‐grafted resazurin molecules. The immobilization of the redox‐labile probes was achieved through chemical modifications on resazurin, followed by bio‐orthogonal click reactions. The lactate detection was demonstrated to be sensitive and specific. The method was incorporated into a single‐cell barcode chip for simultaneous quantification of aerobic glycolysis activities and oncogenic signaling phosphoproteins in cancer. The interplay between glycolysis and oncogenic signaling activities was interrogated on a glioblastoma cell line. Results revealed a drug‐induced oncogenic signaling reliance accompanying shifted metabolic paradigms. A drug combination that exploits this induced reliance exhibited synergistic effects in growth inhibition.
- Subjects
GLIOBLASTOMA multiforme; GLYCOLYSIS; ENZYMES; RESAZURIN; PHOSPHOPROTEINS; CANCER
- Publication
Angewandte Chemie, 2018, Vol 130, Issue 36, p11728
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201803034