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- Title
Folate profile diversity and associated SNPs using genome wide association study in pea.
- Authors
Jha, Ambuj B.; Gali, Krishna K.; Zhang, Haixia; Purves, Randy W.; Tar'an, Bunyamin; Vandenberg, Albert; Warkentin, Thomas D.
- Abstract
Folates are important metabolic cofactors essential for human growth and development. Eighty-five accessions from a pea genome wide association study (GWAS) panel were evaluated for folate profile using ultra-performance liquid chromatography coupled with mass spectrometry. A GWAS was conducted to identify SNP markers associated with the folate profile of pea. Five folates were quantified and the sum of folates ranged from 14 to 55 µg/100 g dry weight. Significant differences (P < 0.001) were observed among the accessions for all folates. The pea accessions were genotyped using genotyping-by-sequencing. After filtering for a read depth of five and minor allele frequency of 0.05, 14,391 SNPs were used for marker-trait association. Five SNP markers were significantly (P < 0.01) associated with the sum of folates. Fifteen, eight, and three SNP markers were associated with 5-methyltetrahydrofolate (5-MTHF), 5-formyltetrahydrofolate, and tetrahydrofolate, respectively. Based on associated SNP markers, an additional 24 accessions were evaluated for folate profile, and those predicted to have a relatively high folate concentration had a significantly greater concentration of 5-MTHF and the sum of folates than those predicted to have a lower concentration. In these accessions, Sc_6992_86348 and Sc_3060_11265 were significantly associated with 5-MTHF and the sum of folates in Saskatoon 2016. Six SNP markers were converted into KASP markers, and these can be used for marker-assisted selection in pea breeding.
- Subjects
SASKATOON (Sask.); PEAS; MASS spectrometry; FOLIC acid; LIQUID chromatography; GENE frequency; HUMAN growth
- Publication
Euphytica, 2020, Vol 216, Issue 2, p1
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-020-2553-8