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- Title
Production and analysis of transgenic wheat ( Triticum aestivum L.) with improved insect resistance by the introduction of cowpea trypsin inhibitor gene.
- Authors
Bi, Rui-Ming; Jia, Hai-Yan; Feng, De-Shun; Wang, Hong-Gang
- Abstract
The cowpea trypsin inhibitor gene ( CpTI) and neomycin phosphotransferase gene ( nptII) were introduced into the embryonic callus cells of immature embryos of wheat elite line Shannong 995604 using Agrobacterium-mediated gene transfer. Independent plantlets were regenerated from kanamycin-resistant calli. PCR and real time PCR analysis, PCR-Southern and Southern blot hybridization indicated that there were three independently-dervied transgenic plants viz. transformed-I, II and III (T-I, T-II and T-III). The segregation of CpTI in the transgenic wheat progenies of T-Iand T-III were consistent with Mendelian inheritance. Resistance to the storage insect pest of wheat viz. the grain moth ( Sitotroga cerealella Olivier) was improved significantly in seeds of the three transgenic wheat T2 lines obtained from T1 PCR-positive plants. The frequency of moth-eaten seed from T-I, T-IIand T-III was reduced 66.76%, 62.48% and 43.59% respectively. The investigation of agronomic traits of the three transgenic wheat T1 PCR-positive plants revealed that the three transgenic lines had excellent agronomic traits. They provide good germplasm resource for wheat genetic improvement.
- Subjects
WHEAT; GRAIN; GRASSES; NEOMYCIN; AMINOGLYCOSIDES; ANTIBACTERIAL agents
- Publication
Euphytica, 2006, Vol 151, Issue 3, p351
- ISSN
0014-2336
- Publication type
Article
- DOI
10.1007/s10681-006-9157-9